Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB1844 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1844, RRID:AB_1572660
- Product name
- AOF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Aof 2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
Forneris F, Binda C, Vanoni MA, Mattevi A, Battaglioli E
FEBS letters 2005 Apr 11;579(10):2203-7
FEBS letters 2005 Apr 11;579(10):2203-7
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
Shi Y, Lan F, Matson C, Mulligan P, Whetstine JR, Cole PA, Casero RA, Shi Y
Cell 2004 Dec 29;119(7):941-53
Cell 2004 Dec 29;119(7):941-53
A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
Hakimi MA, Dong Y, Lane WS, Speicher DW, Shiekhattar R
The Journal of biological chemistry 2003 Feb 28;278(9):7234-9
The Journal of biological chemistry 2003 Feb 28;278(9):7234-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1844). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1844), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)