Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 20813-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HEK-293T cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 20813-1-AP (KDM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 20813-1-AP (KDM1 Antibody) at dilution of 1:50 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 20813-1-AP ( KDM1 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human KDM1 (Product # 20813-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-KDM1 (IP:20813-1-AP, 4ug; Detection:20813-1-AP 1:600) with HeLa cells lysate 2000ug.