Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-11306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LSD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references LSD1 mediated changes in the local redox environment during the DNA damage response.
Duquette ML, Kim J, Shi LZ, Berns MW
PloS one 2018;13(8):e0201907
PloS one 2018;13(8):e0201907
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a LSD1 polyclonal antibody (Product # PA5-11306) in mouse brain tissue lysate (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AOF2 (arrow) using a LSD1 polyclonal antibody (Product # PA5-11306) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a LSD1 polyclonal antibody (Product # PA5-11306). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a LSD1 polyclonal antibody (Product # PA5-11306) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a LSD1 polyclonal antibody (Product # PA5-11306), followed by HRP-conjugated secondary antibody and DAB staining.