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Western blot
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- Antibody Data
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- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-536 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-536, RRID:AB_10001911
- Product name
- Rabbit Polyclonal LSD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-536 is specific for human BHC110-KIAA0601 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Involvement of histone demethylase LSD1 in Blimp-1-mediated gene repression during plasma cell differentiation.
Su ST, Ying HY, Chiu YK, Lin FR, Chen MY, Lin KI
Molecular and cellular biology 2009 Mar;29(6):1421-31
Molecular and cellular biology 2009 Mar;29(6):1421-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LSD1 Antibody [NB100-536] - Detection of Human BHC110/KIAA0601 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: NB100-536 used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
