Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB11290 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11290, RRID:AB_1716933
- Product name
- VDAC1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of VDAC1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase.
In vivo adenoviral transfer of sorcin reverses cardiac contractile abnormalities of diabetic cardiomyopathy.
Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells.
Leary SC, Kaufman BA, Pellecchia G, Guercin GH, Mattman A, Jaksch M, Shoubridge EA
Human molecular genetics 2004 Sep 1;13(17):1839-48
Human molecular genetics 2004 Sep 1;13(17):1839-48
In vivo adenoviral transfer of sorcin reverses cardiac contractile abnormalities of diabetic cardiomyopathy.
Suarez J, Belke DD, Gloss B, Dieterle T, McDonough PM, Kim YK, Brunton LL, Dillmann WH
American journal of physiology. Heart and circulatory physiology 2004 Jan;286(1):H68-75
American journal of physiology. Heart and circulatory physiology 2004 Jan;286(1):H68-75
Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells.
Liu T, Brouha B, Grossman D
Oncogene 2004 Jan 8;23(1):39-48
Oncogene 2004 Jan 8;23(1):39-48
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using VDAC1 polyclonal antibody (Cat # PAB11290) detects a single band at ~32 KDa corresponding to VDAC1 (arrowhead) present in a rat heart tissue lysate.Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.Washes were followed by reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.