Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-C745298 - Provider product page
- Provider
- LSBio
- Product name
- VDAC1 / PORIN Antibody LS-C745298
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Goat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin. Other band(s): Not identified
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: LNCaP Lysate Lane 7: A-172 Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 31 kDa for VDAC\Porin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.