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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- GTX11304 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX11304, RRID:AB_373242
- Product name
- alpha Tubulin antibody [B-5-1-2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Zebrafish
- Host
- Mouse
Submitted references Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.
The role of ubiquitination in hepcidin-independent and hepcidin-dependent degradation of ferroportin.
UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.
Hepcidin-induced internalization of ferroportin requires binding and cooperative interaction with Jak2.
Regulation of mitochondrial iron import through differential turnover of mitoferrin 1 and mitoferrin 2.
Toll-like receptors mediate induction of hepcidin in mice infected with Borrelia burgdorferi.
Iron depletion limits intracellular bacterial growth in macrophages.
Auer TO, Xiao T, Bercier V, Gebhardt C, Duroure K, Concordet JP, Wyart C, Suster M, Kawakami K, Wittbrodt J, Baier H, Del Bene F
eLife 2015 Jun 15;4
eLife 2015 Jun 15;4
The role of ubiquitination in hepcidin-independent and hepcidin-dependent degradation of ferroportin.
De Domenico I, Lo E, Yang B, Korolnek T, Hamza I, Ward DM, Kaplan J
Cell metabolism 2011 Nov 2;14(5):635-46
Cell metabolism 2011 Nov 2;14(5):635-46
UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.
Huang AM, Kao YT, Toh S, Lin PY, Chou CH, Hu HT, Lu CY, Liou JY, Chao SY, Hour TC, Pu YS
Biochemical pharmacology 2011 Jul 1;82(1):35-42
Biochemical pharmacology 2011 Jul 1;82(1):35-42
Hepcidin-induced internalization of ferroportin requires binding and cooperative interaction with Jak2.
De Domenico I, Lo E, Ward DM, Kaplan J
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3800-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3800-5
Regulation of mitochondrial iron import through differential turnover of mitoferrin 1 and mitoferrin 2.
Paradkar PN, Zumbrennen KB, Paw BH, Ward DM, Kaplan J
Molecular and cellular biology 2009 Feb;29(4):1007-16
Molecular and cellular biology 2009 Feb;29(4):1007-16
Toll-like receptors mediate induction of hepcidin in mice infected with Borrelia burgdorferi.
Koening CL, Miller JC, Nelson JM, Ward DM, Kushner JP, Bockenstedt LK, Weis JJ, Kaplan J, De Domenico I
Blood 2009 Aug 27;114(9):1913-8
Blood 2009 Aug 27;114(9):1913-8
Iron depletion limits intracellular bacterial growth in macrophages.
Paradkar PN, De Domenico I, Durchfort N, Zohn I, Kaplan J, Ward DM
Blood 2008 Aug 1;112(3):866-74
Blood 2008 Aug 1;112(3):866-74
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using alpha Tubulin(GTX11304) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HEK293T cells transfected with a HA tagged protein using alpha Tubulin antibody [B-5-1-2] (green), HA antibody (red) and DAPI (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [B-5-1-2] at a concentration of 0.5 ?g/ml.
- Validation comment
- IB
