TA345670

antibody from OriGene

Targeting: TUBA4A FLJ30169, H2-ALPHA, TUBA1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA345670

Provider

OriGene

Product name

Rabbit Polyclonal Anti-TUBA4A Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-TUBA4A Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

TUBA4A

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample Type: 1. Human NT-2 cells (60ug); 2. and 3. mouse brain extracts (80ug); 4. rat brain extract (80ug); Primary Antibody Dilution: 2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20, 000;

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB Suggested Anti-TUBA4A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Placenta

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Amount and Sample Type: 500 ug mouse brain homogenate; Amount of IP Antibody: 6 ug; Primary Antibody: TUBA4A; Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody Dilution: 1: 5000; Gene Name: TUBA4A;

Validation comment

IP

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample Type: Mouse brain stem cells Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594; Secondary Antibody: ilution: 1: 1000; Color/Signal Descriptions: TUBA4A: Red DAPI: Blue; Gene Name: TUBA4A

Validation comment

IHC