Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB120-11304 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11304, RRID:AB_2210210
- Product name
- Mouse Monoclonal alpha Tubulin 4a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes an epitope located at the C-terminal end of the alpha-tubulin isoform in a variety of organisms.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2.3 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo.
PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Heightened muscle inflammation susceptibility may impair regenerative capacity in aging humans.
Ochaba J, Monteys AM, O'Rourke JG, Reidling JC, Steffan JS, Davidson BL, Thompson LM
Neuron 2016 May 4;90(3):507-20
Neuron 2016 May 4;90(3):507-20
PYCR2 Mutations cause a lethal syndrome of microcephaly and failure to thrive.
Zaki MS, Bhat G, Sultan T, Issa M, Jung HJ, Dikoglu E, Selim L, G Mahmoud I, Abdel-Hamid MS, Abdel-Salam G, Marin-Valencia I, Gleeson JG
Annals of neurology 2016 Jul;80(1):59-70
Annals of neurology 2016 Jul;80(1):59-70
Heightened muscle inflammation susceptibility may impair regenerative capacity in aging humans.
Merritt EK, Stec MJ, Thalacker-Mercer A, Windham ST, Cross JM, Shelley DP, Craig Tuggle S, Kosek DJ, Kim JS, Bamman MM
Journal of applied physiology (Bethesda, Md. : 1985) 2013 Sep;115(6):937-48
Journal of applied physiology (Bethesda, Md. : 1985) 2013 Sep;115(6):937-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin 4a Antibody (B-5-1-2) [NB120-11304] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-alpha-Tubulin Clone: B-5-1-2. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) HeLa, 2.) JURKAT, 3.) COS7, 4.) NIH-3T3, 5.) PC-12, 6.) RAT2, 7.) CHO, 8.) MDBK, 9.) MDCK