Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007277-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007277-M01, RRID:AB_548913
- Product name
- Mouse Monoclonal alpha Tubulin 4a Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TUBA1 - tubulin, alpha 1 (testis specific)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi ( Cat # H00007277-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody (M01), clone 2E11 (Cat # H00007277-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody (M01), clone 2E11.Lane 1: TUBA1 transfected lysate(49.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - TUBA1 monoclonal antibody (M01), clone 2E11. Analysis of TUBA1 expression in different Cell lines and Human Tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - TUBA1 monoclonal antibody (M01), clone 2E11. Analysis of TUBA1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.