- MA5-32738 - Invitrogen Antibodies TUBA4A antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical analysis of alpha Tubulin in Hela cells using a alpha Tubulin Monoclonal antibody (Product # MA5-32738) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical analysis of TUBA4A in NIH-3T3 cells using a TUBA4A Monoclonal antibody (Product # MA5-32738) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical analysis of TUBA4A in SW480 cells using a TUBA4A Monoclonal antibody (Product # MA5-32738) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence of TUBA4A was performed using HAP1 wild-type and TUBA4A KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the TUBA4A recombinant monoclonal antibody (Product # MA5-32738) at a 1:1,000 dilution overnight at 4°C. Secondary antibody incubation was performed using 1 µg/mL of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody (Product # A21429) together with DAPI for 1 hr. Imaging was performed with a 40X oil objective and analysis was performed using Image J. Cell image represents a single focal plane; WT and KO cells are outlined with a yellow (WT) or magenta (KO) dashed line. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of TUBA4A of paraffin-embedded Human tonsil tissue using a TUBA4A Monoclonal antibody (Product #MA5-32738). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of TUBA4A of paraffin-embedded Human thyroid tissue using a TUBA4A Monoclonal antibody (Product #MA5-32738). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of TUBA4A of paraffin-embedded Mouse brain tissue using a TUBA4A Monoclonal antibody (Product #MA5-32738). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of TUBA4A of paraffin-embedded Human placenta tissue using a TUBA4A Monoclonal antibody (Product #MA5-32738). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TUBA4A of paraffin-embedded Mouse testes tissue using a TUBA4A Monoclonal antibody (Product #MA5-32738). Counter stained with hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow Cytometric analysis of TUBA4A in Hela cells using a TUBA4A Monoclonal Antibody (Product # MA5-32738) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

MA5-32738