AP12740c

antibody from Abgent

Targeting: SIX5 DMAHP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12740c

Provider

Abgent

Proper citation

Abgent Cat#AP12740c, RRID:AB_10820673

Product name

SIX5 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SIX5 Antibody (Center) (Cat. #AP12740c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of Hela cell stained with SIX5 Antibody (Center)(Cat#AP12740c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SIX5 Antibody (Center) (Cat. #AP12740c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50