PA5-75417

antibody from Invitrogen Antibodies

Targeting: SIX5 DMAHP

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-75417

Provider

Invitrogen Antibodies

Product name

SIX5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression.

Yang C, Liu H
Annals of translational medicine 2022 Jul;10(13):752

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

The assembly of EYA3-SIX5-p300 complex in vivo and in vitro . (A) EYA3 pulled down both SIX5 and p300 in cancerous biopsies. Three independent cancerous were mixed with equal weights and were then immunoprecipitated using anti-EYA3 (or IgG, negative control) conjugated agarose. The output complexes were probed with antibodies as indicated in the figure. (B) EYA3 pulled down both SIX5 and p300 in HCEC-1CT cells expressing pCDNA3-Flag-EYA3. (C,D) Detection of direct interaction of EYA3-p300, EYA3-SIX5, p300-SIX5, and p300-EYA3 in vitro . Different combinations of plasmids as shown in the figure were transfected into HCEC-1CT cells, followed by immunoprecipitation with anti-Flag- and anti-Myc-agarose. The outputs were probed using anti-Flag and anti-Myc. (C) Interactions of EYA3-p300 and EYA3-SIX5. (D) Interactions of p300-SIX5 and p300-EYA3. (E) Schematic assembly of the EYA3-SIX5-p300 complex. IgG, immunoglobulin G.