PA5-35938

antibody from Invitrogen Antibodies

Targeting: NOLC1 KIAA0035, NOPP130, NOPP140, P130, Srp40

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35938

Provider

Invitrogen Antibodies

Product name

NOLC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HCT116, K562, HL-60. Predicted reactivity: Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NOLC1 Polyclonal Antibody detects NOLC1 protein by western blot analysis. A. 30 µg H1299 whole cell lysate/extract. B. 30 µg HCT116 whole cell lysate/extract.5 % SDS-PAGE. NOLC1 Polyclonal Antibody (Product # PA5-35938) dilution: 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NOLC1 Polyclonal Antibody detects NOLC1 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract. B. 30 µg HL-60 whole cell lysate/extract.5 % SDS-PAGE. NOLC1 Polyclonal Antibody (Product # PA5-35938) dilution: 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NOLC1 Polyclonal Antibody detects NOLC1 protein at cytoplasm and nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOLC1 protein stained by NOLC1 Polyclonal Antibody (Product # PA5-35938) diluted at 1:500. Blue: Hoechst 33342 staining.