PA5-78849

antibody from Invitrogen Antibodies

Targeting: AZIN2 ADC, KIAA1945, ODC-p, ODC1L, ODCp

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78849

Provider

Invitrogen Antibodies

Product name

ADC Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADC in rat brain extract (lane 1), mouse brain extract (lane 2) and HeLa whole cell lysate (lane 3). Sample was incubated with ADC polyclonal antibody (Product # PA5-78849) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADC in rat brain extract (lane 1), mouse brain extract (lane 2) and HeLa whole cell lysate (lane 3). Sample was incubated with ADC polyclonal antibody (Product # PA5-78849) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ADC on paraffin-embedded mouse brain tissue. Sample was incubated with ADC polyclonal antibody (Product# PA5-78849) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ADC on paraffin-embedded rat brain tissue. Sample was incubated with ADC polyclonal antibody (Product# PA5-78849) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of AZIN2 in paraffin-embedded section of human testicular cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-AZIN2 antibody (Product # PA5-78849) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.