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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- RZ1114 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Zebrafish Gbf1 Antibody / Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Zebrafish
- Host
- Rabbit
- Vial size
- 100 ug
- Concentration
- Lyophilized
- Storage
- After reconstitution, the Zebrafish Gbf1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Gbf1 protein using Zebrafish Gbf1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~208 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Gbf1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish heart tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish kidney tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish brain tissue with Zebrafish Gbf1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
