PA5-95792

antibody from Invitrogen Antibodies

Targeting: CHDH

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95792

Provider

Invitrogen Antibodies

Product name

CHDH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LHPALSRTNL KAEAETLVSR VLFEGTRAVG VEYVKNGQSH RAYASKEVIL SGGAINSPQL LMLSGIGNAD DLKKLGIPVV CHLPGVGQNL QDHLEIYIQQ ACTRPITLHS AQKPLRKVCI GLEWLWKFTG EGATAHLETG GFIRSQPGVP HPDIQFHFLP SQVIDHGRVP TQQEAYQVHV GPMRGTSVGW LKLRSANPQD HPVIQPNYLS TETDIEDFRL CVKLTREIFA QEALAPFRGK ELQPGSHIQS DKEIDAFVRA KADSAYHPSC TCKMGQPSDP TAVVDPQTRV LGVENLRVVD ASIMPSMVSG NLNAPTIMIA EKAADIIKGQ PALWDKDVPV YKPRTLATQR; Positive Samples: 293T, HepG2, Mouse thymus, Mouse kidney, Mouse liver, Mouse pancreas, Mouse testis, Rat kidney; Cellular Location: Mitochondrion inner membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHDH in extracts of various cell lines. Samples were incubated with CHDH Polyclonal antibody (Product # PA5-95792) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHDH in paraffin-embedded human colon. Samples were incubated with CHDH Polyclonal antibody (Product # PA5-95792) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHDH in paraffin-embedded mouse kidney. Samples were incubated with CHDH Polyclonal antibody (Product # PA5-95792) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHDH in paraffin-embedded rat kidney. Samples were incubated with CHDH Polyclonal antibody (Product # PA5-95792) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHDH in paraffin-embedded rat kidney. Samples were incubated with CHDH Polyclonal antibody (Product # PA5-95792) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.