Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX112268 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112268, RRID:AB_1950163
- Product name
- EED antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody (GTX112268) diluted at 1:500.
- Protocol
- Protocol
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EED antibody detects EED protein by Western blot analysis.A. 30 μg H1299 whole cell lysate/extractB. 30 μg HCT116 whole cell lysate/extract10 % SDS-PAGEEED antibody (GTX112268) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using EED(GTX112268) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS Xenograft, using EED(GTX112268) antibody at 1:100 dilution.