GTX112268

antibody from GeneTex

Targeting: EED HEED, WAIT-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX112268

Provider

GeneTex

Proper citation

GeneTex Cat#GTX112268, RRID:AB_1950163

Product name

EED antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody (GTX112268) diluted at 1:500.

Protocol

Protocol

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

41129

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

EED antibody detects EED protein by Western blot analysis.A. 30 μg H1299 whole cell lysate/extractB. 30 μg HCT116 whole cell lysate/extract10 % SDS-PAGEEED antibody (GTX112268) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using EED(GTX112268) antibody at 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SAS Xenograft, using EED(GTX112268) antibody at 1:100 dilution.