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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-72224 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-EED Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Eed in paraffin-embedded mouse spleen tissue. Samples were fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature. Antigen retrieval was performed by heat mediation with a citrate buffer (pH6). Samples were then incubated with Eed polyclonal antibody (Product # PA5-72224) using a dilution of 1:25 for 1 hours at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
