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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ACC-103-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 3) and humanK562 chronic myelogeneous leukemia (lanes 2 and 4) lysates: - 1,2. Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody (#ACC-103), (1:200).3,4. Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody , preincubated with CACNA2D3/Cav alpha 2 delta 3 (extracellular) Blocking Peptide (#BLP-CC103).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 3) and humanK562 chronic myelogeneous leukemia (lanes 2 and 4) lysates: - 1,2. Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody (#ACC-103), (1:200).3,4. Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody , preincubated with CACNA2D3/Cav alpha 2 delta 3 (extracellular) Blocking Peptide (#BLP-CC103).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of CaV alpha 2 delta 3 in rat PC12 cells - Cell surface detection of CaV alpha 2 delta 3 in intact living PC12 cells. Extracellular staining of cells using Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody (#ACC-103), (1:25) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of CaV alpha 2 delta 3 in rat hippocampus and cortex - Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-CACNA2D3 (CaV alpha 2 delta 3) (extracellular) Antibody (#ACC-103). In both areas CaV alpha 2 delta 3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).
