Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- RQ7660 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HP59 Antibody / Sialin / SLC17A5
- Antibody type
- Polyclonal
- Description
- This highly specific HP59 antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the HP59 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human 293T, 3) monkey COS-7, 4) human ThP-1, 5) rat NRK and 6) mouse HBZY cell lysate with HP59 antibody. Predicted molecular weight ~55 kDa and 31 kDa (two isoform) which may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thyroid cancer tissue with HP59 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.