PA5-120396

antibody from Invitrogen Antibodies

Targeting: ME3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120396

Provider

Invitrogen Antibodies

Product name

ME3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HT-29, 293T, Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat heart, Rat kidney. The target is usually found in the following locations: Mitochondrion matrix. Immunogen sequence: IRHIPDEIFL LTAEQIAQEV SEQHLSQGRL YPPLSTIRDV SLRIAIKVLD YAYKHNLASY YPEPKDKEAF VRSLVYTPDY DSFTLDSYTW PKEAMNVQTV

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.64 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ME3 in various cell lines using a ME3 Polyclonal antibody (Product # PA5-120396) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ME3 in L929 cells using a ME3 Polyclonal antibody (Product # PA5-120396). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ME3 in human colon carcinoma using a Polyclonal antibody (Product # PA5-120396) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ME3 in human colon carcinoma using a Polyclonal antibody (Product # PA5-120396) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ME3 in human colon carcinoma using a Polyclonal antibody (Product # PA5-120396) at a dilution of 1:100.