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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB645 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Wnt-5a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt-5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis.
Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells.
ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling.
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition.
Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
Oncogenic effects of WNT5A in Epstein-Barr virus‑associated nasopharyngeal carcinoma.
Wnt5a promotes inflammatory responses via nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
Interleukin-6 drives melanoma cell motility through p38α-MAPK-dependent up-regulation of WNT5A expression.
Wnt5a/β-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
Asem M, Young AM, Oyama C, Claure De La Zerda A, Liu Y, Yang J, Hilliard TS, Johnson J, Harper EI, Guldner I, Zhang S, Page-Mayberry T, Kaliney WJ, Stack MS
Cancer research 2020 Mar 1;80(5):1156-1170
Cancer research 2020 Mar 1;80(5):1156-1170
Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
Hasan MK, Rassenti L, Widhopf GF 2nd, Yu J, Kipps TJ
Leukemia 2019 Mar;33(3):653-661
Leukemia 2019 Mar;33(3):653-661
Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells.
Huang TC, Lee PT, Wu MH, Huang CC, Ko CY, Lee YC, Lin DY, Cheng YW, Lee KH
PloS one 2017;12(8):e0181034
PloS one 2017;12(8):e0181034
ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling.
Vesel M, Rapp J, Feller D, Kiss E, Jaromi L, Meggyes M, Miskei G, Duga B, Smuk G, Laszlo T, Karner I, Pongracz JE
Respiratory research 2017 Mar 24;18(1):52
Respiratory research 2017 Mar 24;18(1):52
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD.
Baarsma HA, Skronska-Wasek W, Mutze K, Ciolek F, Wagner DE, John-Schuster G, Heinzelmann K, Günther A, Bracke KR, Dagouassat M, Boczkowski J, Brusselle GG, Smits R, Eickelberg O, Yildirim AÖ, Königshoff M
The Journal of experimental medicine 2017 Jan;214(1):143-163
The Journal of experimental medicine 2017 Jan;214(1):143-163
WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition.
Prasad CP, Chaurasiya SK, Guilmain W, Andersson T
Journal of experimental & clinical cancer research : CR 2016 Sep 13;35(1):144
Journal of experimental & clinical cancer research : CR 2016 Sep 13;35(1):144
Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
Fuster JJ, Zuriaga MA, Ngo DT, Farb MG, Aprahamian T, Yamaguchi TP, Gokce N, Walsh K
Diabetes 2015 Apr;64(4):1235-48
Diabetes 2015 Apr;64(4):1235-48
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
MacMillan CD, Leong HS, Dales DW, Robertson AE, Lewis JD, Chambers AF, Tuck AB
Scientific reports 2014 Sep 10;4:6315
Scientific reports 2014 Sep 10;4:6315
Oncogenic effects of WNT5A in Epstein-Barr virus‑associated nasopharyngeal carcinoma.
Yap LF, Ahmad M, Zabidi MM, Chu TL, Chai SJ, Lee HM, Lim PV, Wei W, Dawson C, Teo SH, Khoo AS
International journal of oncology 2014 May;44(5):1774-80
International journal of oncology 2014 May;44(5):1774-80
Wnt5a promotes inflammatory responses via nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
Zhao Y, Wang CL, Li RM, Hui TQ, Su YY, Yuan Q, Zhou XD, Ye L
The Journal of biological chemistry 2014 Jul 25;289(30):21028-39
The Journal of biological chemistry 2014 Jul 25;289(30):21028-39
Interleukin-6 drives melanoma cell motility through p38α-MAPK-dependent up-regulation of WNT5A expression.
Linnskog R, Jönsson G, Axelsson L, Prasad CP, Andersson T
Molecular oncology 2014 Dec;8(8):1365-78
Molecular oncology 2014 Dec;8(8):1365-78
Wnt5a/β-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
The Journal of investigative dermatology 2014 Aug;134(8):2183-2191
The Journal of investigative dermatology 2014 Aug;134(8):2183-2191
Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
Barbolina MV, Burkhalter RJ, Stack MS
The Biochemical journal 2011 Jul 1;437(1):1-12
The Biochemical journal 2011 Jul 1;437(1):1-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Wnt-5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Wnt-5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
