66543-1-Ig

antibody from Proteintech Group

Targeting: SIRT4 SIR2L4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Antibody Data

Product number

66543-1-Ig

Provider

Proteintech Group

Product name

SIRT4 antibody

Antibody type

Monoclonal

Description

KD/KO validated SIRT4 antibody (Cat. #66543-1-Ig) is a mouse monoclonal antibody that shows reactivity with Human, mouse, rat, pig and has been validated for the following applications: WB, ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1D5A2

Vial size

20ul, 150ul

References

Submitted references

SIRT4 regulates antiviral and autoimmune responses by promoting cGAS-mediated signaling pathways.

Yang B, Zhang Y, Wang S, Wu Y, Diao Z, Zhang Q, Lu C, Shen M, Zhang X, Ma S, Yang C, Pei J, Xing H, Liang Y, Wang J
EMBO reports 2026 Mar;27(5):1228-1253

---

Ferroptosis promotes sepsis-related lung injury via endothelial cell senescence.

Yao Y, Wang S, Shi W, Yang L, Tian Y, Wang D, Xia D
Cellular signalling 2025 Nov;135:112018

---

H(2)S remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes.

Hanna DA, Chen B, Shah YM, Khalimonchuk O, Cunniff B, Banerjee R
The Journal of biological chemistry 2025 May;301(5):108433

---

Sirt4 Overexpression Modulates the JAK2/STAT3 and PI3K/AKT/mTOR Axes to Alleviate Sepsis-Induced Acute Lung Injury.

Xie C, Wang T, Liu A, Huang B, Zeng W, Li Z, Peng S, Wu S
Cell biochemistry and biophysics 2025 Jun;83(2):1785-1798

---

The SIRT6/BAP1/xCT signaling axis mediates ferroptosis in cisplatin-induced AKI.

Yang S, Chen L, Din S, Ye Z, Zhou X, Cheng F, Li W
Cellular signalling 2025 Jan;125:111479

---

ASIC1a Promotes nucleus pulposus derived stem cells apoptosis through modulation of SIRT3-dependent mitochondrial redox homeostasis in intervertebral disc degeneration.

Zhang ZG, Kang L, Zhou LP, Wang YX, Jia CY, Zhao CH, Zhang B, Wang JQ, Zhang HQ, Zhang RJ, Shen CL
Redox report : communications in free radical research 2025 Dec;30(1):2504120

---

Sirtuin 1-mediated recruitment of non-structural protein 1 to glucocorticoid receptor induces glucocorticoid insensitivity post RSV infection.

Che S, Long X, Mo S, Jiang Z, Xie X, Liu E, Xie J
International journal of biological macromolecules 2025 Aug;320(Pt 1):145811

---

Silencing KLF6 Alleviates Cigarette Smoke Extract-Induced Mitochondrial Dysfunction in Bronchial Epithelial Cells by SIRT4 Upregulation.

Wan M, Wang C, Cui J, Xia Q, Zhang L
International journal of chronic obstructive pulmonary disease 2024;19:815-828

---

H (2) S remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes.

Hanna DA, Chen B, Shah YM, Khalimonchuk O, Cunniff B, Banerjee R
bioRxiv : the preprint server for biology 2024 Nov 3;

---

USP14 modulates cell pyroptosis and ameliorates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing SIRT3.

Zhang Z, Jin B, Zhang Y, Yang M, Wang C, Zhu Y, Li T, Lin J, Yang M, Cheng Y, Xu S, He K, Xu J, Mi Y, Jiang J, Sun Z
Free radical biology & medicine 2024 Nov 20;225:741-757

---

The SP1/SIRT1/ACLY signaling axis mediates fatty acid oxidation in renal ischemia-reperfusion-induced renal fibrosis.

Wu H, Wang L, Kang P, Zhou X, Li W, Xia Z
International immunopharmacology 2024 May 10;132:112002

---

β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice.

Gu Y, Gao L, He J, Luo M, Hu M, Lin Y, Li J, Hou T, Si J, Yu Y
Food & function 2024 Mar 18;15(6):3199-3213

---

Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy.

Qu H, Liu X, Zhu J, He N, He Q, Zhang L, Wang Y, Gong X, Xiong X, Liu J, Wang C, Yang G, Yang Q, Luo G, Zhu Z, Zheng Y, Zheng H
iScience 2024 Jun 21;27(6):109796

---

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma.

Liang J, Zhou C, Zhang C, Liang S, Zhou Z, Zhou Z, Wu C, Zhao H, Meng X, Zou F, Yu C, Cai S
International immunopharmacology 2024 Jan 25;127:111328

---

Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hydroxymethylcytosine Loss.

Yi Y, Wang Y, Wu Y, Liu Y
Laboratory investigation; a journal of technical methods and pathology 2024 Feb;104(2):100268

---

Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer.

Meng L, Yang J, Gao Y, Cao Q, Jiang S, Xiao Y, Wang H, Liu W, Yuan A, Li Y, Huang H
ACS nano 2024 Feb 6;18(5):4360-4375

---

Sirt6 regulates the proliferation of neural precursor cells and cortical neurogenesis in mice.

Wei Y, Wang X, Ma Z, Xiang P, Liu G, Yin B, Hou L, Shu P, Liu W, Peng X
iScience 2024 Feb 16;27(2):108706

---

Biodegradable Zn-5Dy Alloy with Enhanced Osteo/Angio-Genic Activity and Osteointegration Effect via Regulation of SIRT4-Dependent Mitochondrial Function.

Han Y, Tong X, Zhou R, Wang Y, Chen Y, Chen L, Hong X, Wu L, Lin Z, Zhang Y, Zhang X, Hu C, Li B, Ping Y, Cao Z, Ye Z, Song Z, Li Y, Wen C, Zhou Y, Lin J, Huang S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Apr;11(13):e2307812

---

Korean Red Ginseng-Induced SIRT3 Promotes the Tom22-HIF-1α Circuit in Normoxic Astrocytes.

Kim H, Moon S, Lee D, Park J, Kim CH, Kim YM, Choi YK
Cells 2023 May 30;12(11)

---

Metrnl Alleviates Lipid Accumulation by Modulating Mitochondrial Homeostasis in Diabetic Nephropathy.

Zhou Y, Liu L, Jin B, Wu Y, Xu L, Chang X, Hu L, Wang G, Huang Y, Song L, Zhang T, Wang Y, Xiao Y, Zhang F, Shi M, Liu L, Wang T, Yan R, Guo B
Diabetes 2023 May 1;72(5):611-626

---

miR-124-3p improves mitochondrial function of renal tubular epithelial cells in db/db mice.

Liang L, Wo C, Yuan Y, Cao H, Tan W, Zhou X, Wang D, Chen R, Shi M, Zhang F, Xiao Y, Liu L, Zhou Y, Zhang T, Wang Y, Guo B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Mar;37(3):e22794

---

AGER1 deficiency-triggered ferroptosis drives fibrosis progression in nonalcoholic steatohepatitis with type 2 diabetes mellitus.

Gong Y, Liu Z, Zhang Y, Zhang J, Zheng Y, Wu Z
Cell death discovery 2023 Jun 6;9(1):178

---

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice.

Chen JW, Shan TK, Wei TW, Jiang QQ, Du C, Gu LF, Yang TT, Zhou LH, Wang SB, Bao YL, Wang H, Ji Y, Xie LP, Gu AH, Sun CQ, Wang QM, Wang LS
Archives of toxicology 2023 Dec;97(12):3209-3226

---

CoCl(2) -triggered pseudohypoxic stress induces proteasomal degradation of SIRT4 via polyubiquitination of lysines K78 and K299.

Hampel N, Georgy J, Mehrabipour M, Lang A, Lehmkuhl I, Scheller J, Ahmadian MR, Floss DM, Piekorz RP
FEBS open bio 2023 Dec;13(12):2187-2199

---

Amino acids downregulate SIRT4 to detoxify ammonia through the urea cycle.

Hu SH, Feng YY, Yang YX, Ma HD, Zhou SX, Qiao YN, Zhang KH, Zhang L, Huang L, Yuan YY, Lin Y, Zhang XY, Li Y, Li HT, Zhao JY, Xu W, Zhao SM
Nature metabolism 2023 Apr;5(4):626-641

---

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's mouse model.

Weng H, Song W, Fu K, Guan Y, Cai G, Huang E, Chen X, Zou H, Ye Q
Frontiers in neuroscience 2022;16:1035444

---

Rosmarinic Acid Inhibits Mitochondrial Damage by Alleviating Unfolded Protein Response.

Cai G, Lin F, Wu D, Lin C, Chen H, Wei Y, Weng H, Chen Z, Wu M, Huang E, Ye Z, Ye Q
Frontiers in pharmacology 2022;13:859978

---

Correlation between the reduction in hippocampal SirT2 expression and depressive-like behaviors and neurological abnormalities in olfactory bulbectomized mice.

Takahashi K, Kurokawa K, Hong L, Miyagawa K, Mochida-Saito A, Takeda H, Tsuji M
Neuroscience research 2022 Sep;182:76-80

---

Omentin1 ameliorates myocardial ischemia-induced heart failure via SIRT3/FOXO3a-dependent mitochondrial dynamical homeostasis and mitophagy.

Hu J, Liu T, Fu F, Cui Z, Lai Q, Zhang Y, Yu B, Liu F, Kou J, Li F
Journal of translational medicine 2022 Oct 4;20(1):447

---

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

Li Y, Yang L, Hu F, Xu J, Ye J, Liu S, Wang L, Zhuo M, Ran B, Zhang H, Ye J, Xiao J
ACS applied materials & interfaces 2022 Jun 8;14(22):25155-25172

---

Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.

Sivalingam K, Doke M, Khan MA, Samikkannu T
Scientific reports 2021 Oct 29;11(1):21335

---

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.

Chen H, Kang J, Zhang F, Yan T, Fan W, He H, Huang F
The international journal of biochemistry & cell biology 2021 May;134:105962

---

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Cui W, Wu X, Shi Y, Guo W, Luo J, Liu H, Zheng L, Du Y, Wang P, Wang Q, Feng D, Ge S, Qu Y
Cell proliferation 2021 Feb;54(2):e12964

---

SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.

Liu M, Qin Y, Hu Q, Liu W, Ji S, Xu W, Fan G, Ye Z, Zhang Z, Xu X, Yu X, Zhuo Q
Cancer letters 2021 Feb 28;499:265-278

---

SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.

Leng S, Huang W, Chen Y, Yang Y, Feng D, Liu W, Gao T, Ren Y, Huo M, Zhang J, Yang Y, Wang Y
Cell death and differentiation 2021 Dec;28(12):3329-3343

---

SIRT4 is the molecular switch mediating cellular proliferation in colorectal cancer through GLS mediated activation of AKT/GSK3β/CyclinD1 pathway.

Cui Y, Bai Y, Yang J, Yao Y, Zhang C, Liu C, Shi J, Li Q, Zhang J, Lu X, Zhang Y
Carcinogenesis 2021 Apr 17;42(3):481-492

---

miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.

Cui Y, Yang J, Bai Y, Zhang Y, Yao Y, Zheng T, Liu C, Wu F
Journal of Cancer 2020;11(21):6337-6347

---

Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.

Drews L, Zimmermann M, Westhoff P, Brilhaus D, Poss RE, Bergmann L, Wiek C, Brenneisen P, Piekorz RP, Mettler-Altmann T, Weber APM, Reichert AS
Disease models & mechanisms 2020 Nov 4;13(10)

---

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8

---

SIRT4 enhances the sensitivity of ER-positive breast cancer to tamoxifen by inhibiting the IL-6/STAT3 signal pathway.

Xing J, Li J, Fu L, Gai J, Guan J, Li Q
Cancer medicine 2019 Nov;8(16):7086-7097