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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31428 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, 3XFlag-human TRIM8-transfected 293T. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (91%), Cat (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRIM8 Polyclonal Antibody (Product # PA5-31428). Sample (30 µg of whole cell lysate). Lane A: HepG2 . 7.5% SDS PAGE. TRIM8 Polyclonal Antibody (Product # PA5-31428) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM8 Polyclonal Antibody detects GERP protein by western blot analysis. Non-transfected (-) and -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM8 Polyclonal Antibody (Product # PA5-31428) diluted by 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using GERP (Product # PA5-31428) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
