MA5-42566

antibody from Invitrogen Antibodies

Targeting: LRRN3 FIGLER5, FLJ11129, NLRR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42566

Provider

Invitrogen Antibodies

Product name

LRRN3 Monoclonal Antibody (A4-6-3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: SHSY5Ycell lysate, rat testis tissue lysate, F9. Subcellular Location: Membrane

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgM

Antibody clone number

A4-6-3

Vial size

100 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRRN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. LRRN3 Monoclonal Antibody (Product # MA5-42566) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY5Ycell lysate. Lane 2: Rat testis tissue lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with LRRN3 Monoclonal Antibody (Product # MA5-42566) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of LRRN3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with LRRN3 Monoclonal Antibody (Product # MA5-42566) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).