14447-1-AP

antibody from Proteintech Group

Targeting: CYP17A1 CPT7, CYP17, P450C17, S17AH

Provider product page for 14447-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [40]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 14447-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14447-1-AP, RRID:AB_2292527
Product name: CYP17A1 antibody
Antibody type: Polyclonal
Description: CYP17A1 antibody (Cat. #14447-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CYP17A1 protein structure - 14447-1-AP shown in red.

Submitted references Androgen synthesis cell-specific CREBZF deficiency alters adrenal cortex steroid secretion and develops behavioral abnormalities in adult male mice.

Niu H, Li C, Zhang H, Liu H, Shang C, Jia Y, Wuenjiya, Li Z, Wang A, Jin Y, Lin P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 May 15;38(9):e23650

Effects of sub-chronic exposure to microcystin-LR on the endocrine system of male rats.

Wang YT, Wu QH, Chen L, Giesy JP, Xu LL, Xu WL, He J, Shi T, Liu YQ, Xiao SM, Wang YK, Chen F, Chen Y, Xu NH, Ge YL, Chu L, Yan YZ, Chen J, Xie P
The Science of the total environment 2024 Jan 1;906:166839

Bulk RNA-sequencing, single-cell RNA-sequencing analysis, and experimental validation reveal iron metabolism-related genes CISD2 and CYP17A1 are potential diagnostic markers for recurrent pregnancy loss.

He YB, Han L, Wang C, Fang J, Shang Y, Cai HL, Zhou Q, Zhang ZZ, Chen SL, Li JY, Liu YL
Gene 2024 Apr 5;901:148168

Involvement of p38 MAPK in Leydig cell aging and age-related decline in testosterone.

Luo D, Qi X, Xu X, Yang L, Yu C, Guan Q
Frontiers in endocrinology 2023;14:1088249

Paternal cadmium exposure affects estradiol synthesis by impairing intracellular cholesterol homeostasis and mitochondrial function in offspring female mice.

Zhang Y, Zhou J, Zeng L, Xiong Y, Wang X, Xiang W, Su P
Ecotoxicology and environmental safety 2023 Sep 15;263:115280

N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway.

Jing YX, Li HX, Yue F, Li YM, Yu X, He JJ, Zhang XH
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 2023 Oct 26;39(1):2276167

Sea cucumber peptides positively regulate sexual hormones in male mice with acute exhaustive swimming: possibly through the Ca(2+)/PKA signaling pathway.

Luo X, Liu W, Zheng B, Zheng Y, Zhao M, Feng F, Liu L
Food & function 2023 Nov 13;14(22):10188-10203

Salt-inducible kinases regulate androgen synthesis in theca cells by enhancing CREB signaling.

Rodriguez Esquivel M, Hayes E, Lakomy O, Hassan M, Foretz M, Stocco C
Molecular and cellular endocrinology 2023 Nov 1;577:112030

Bisphenol S induces oxidative stress-mediated impairment of testosterone synthesis by inhibiting the Nrf2/HO-1 signaling pathway.

Wang YX, Dai W, Li YZ, Wu ZY, Kan YQ, Zeng HC, He QZ
Journal of biochemical and molecular toxicology 2023 Mar;37(3):e23273

Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis.

Milosevic A, Lavrnja I, Savic D, Milosevic K, Skuljec J, Bjelobaba I, Janjic MM
Cells 2023 Mar 30;12(7)

The molecular mechanism of miR-96-5p in the pathogenesis and treatment of polycystic ovary syndrome.

Liu Y, Zhang S, Chen L, Huang X, Wang M, Ponikwicka-Tyszko D, Rahman NA, Wolczynski S, Yao B, Li X
Translational research : the journal of laboratory and clinical medicine 2023 Jun;256:1-13

Low-Dose Sodium Salicylate Promotes Ovulation by Regulating Steroids via CYP17A1.

Li T, Ren X, Li T, Yu L, Teng M, Zheng Y, Lei A
International journal of molecular sciences 2023 Jan 30;24(3)

Persistence of foetal testicular features in patients with defective androgen signalling.

Al-Sharkawi M, Calonga-Solís V, Dressler FF, Busch H, Hiort O, Werner R
European journal of endocrinology 2023 Jan 10;188(1)

Luteinizing hormone receptor promotes angiogenesis in ovarian endothelial cells of Macaca fascicularis and Homo sapiens†.

Lund M, Pearson AC, Sage MAG, Duffy DM
Biology of reproduction 2023 Feb 13;108(2):258-268

SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability.

Lettieri A, Oleari R, van den Munkhof MH, van Battum EY, Verhagen MG, Tacconi C, Spreafico M, Paganoni AJJ, Azzarelli R, Andre' V, Amoruso F, Palazzolo L, Eberini I, Dunkel L, Howard SR, Fantin A, Pasterkamp RJ, Cariboni A
Nature communications 2023 Dec 7;14(1):8097

Periportal hepatocyte proliferation at midgestation governs maternal glucose homeostasis in mice.

Kozuki S, Kabata M, Sakurai S, Iwaisako K, Nishimura T, Toi M, Yamamoto T, Toyoshima F
Communications biology 2023 Dec 4;6(1):1226

Effect of iodoacetic acid on the reproductive system of male mice.

Liang Y, Huang X, Fang L, Wang M, Yu C, Guan Q
Frontiers in pharmacology 2022;13:958204

Astragalus polysaccharide protects sepsis model rats after cecum ligation and puncture.

Li J, Zhao J, Chai Y, Li W, Liu X, Chen Y
Frontiers in bioengineering and biotechnology 2022;10:1020300

Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.

Wang Q, Yan Q, Nan J, Wang J, Zhang Y, Zhao X
The Journal of steroid biochemistry and molecular biology 2022 Oct;223:106135

Excess dietary zinc drives a Cushing's-like syndrome in ovariectomized mice - Implications for postmenopausal obesity.

Zhao Y, Wang Y, Hu L, Chen H, Xiao X, Li Z, Feng M, Yang P, Liu P, Zhang S, Xiao G, Liu J, Shen J, Bai X
Biochemical and biophysical research communications 2022 Nov 19;630:101-111

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Jin H, Yan M, Pan C, Liu Z, Sha X, Jiang C, Li L, Pan M, Li D, Han X, Ding J
Particle and fibre toxicology 2022 Feb 17;19(1):13

A Novel SEMA3G Mutation in Two Siblings Affected by Syndromic GnRH Deficiency.

Oleari R, André V, Lettieri A, Tahir S, Roth L, Paganoni A, Eberini I, Parravicini C, Scagliotti V, Cotellessa L, Bedogni F, De Martini LB, Corridori MV, Gulli S, Augustin HG, Gaston-Massuet C, Hussain K, Cariboni A
Neuroendocrinology 2021;111(5):421-441

Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary.

Alam J, Ogawa K
Reproductive biology 2021 Sep;21(3):100511

Effect of the spatial-temporal specific theca cell Cyp17 overexpression on the reproductive phenotype of the novel TC17 mouse.

Secchi C, Belli M, Harrison TNH, Swift J, Ko C, Duleba AJ, Stupack D, Chang RJ, Shimasaki S
Journal of translational medicine 2021 Oct 15;19(1):428

Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells.

Su Y, Tian Z, Qi X, Luo D, Liu L, Liu S, Zheng D, Wei F, He Z, Guan Q
Nutrition & metabolism 2021 May 26;18(1):53

WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.

Habara O, Logan CY, Kanai-Azuma M, Nusse R, Takase HM
Development (Cambridge, England) 2021 May 1;148(9)

Expression and localisation of ephrin-B1, EphB2, and EphB4 in the mouse testis during postnatal development.

Gofur MR, Alam J, Ogawa K
Reproductive biology 2020 Sep;20(3):321-332

3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.

Lu J, Lu J, Chen Y, Feng Z, Liu S, Guan S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Oct;144:111575

Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress.

Peng Y, Yang X, Luo X, Liu C, Cao X, Wang H, Guo L
Biological research 2020 Oct 27;53(1):50

Features of the fetal gonad in androgen synthesis in the postpubertal testis are preserved in complete androgen insensitivity syndrome due to a novel genetic splice site donor variant in androgen receptor gene intron 1.

Jarzabek K, Koda M, Chrusciel M, Kanczuga-Koda L, Sobczynska-Tomaszewska A, Rahman NA, Wolczynski S
The Journal of steroid biochemistry and molecular biology 2019 Oct;193:105420

The Involvement of Granulosa Cells in the Regulation by Gonadotropins of Cyp17a1 in Theca Cells.

Kakuta H, Iguchi T, Sato T
In vivo (Athens, Greece) 2018 Nov-Dec;32(6):1387-1401

Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring.

Tian H, Chen S, Leng Y, Li T, Li Z, Chen H, Zhang Q
Environmental toxicology 2018 Mar;33(3):351-360

Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease.

Jokela H, Hakkarainen J, Kätkänaho L, Pakarinen P, Ruohonen ST, Tena-Sempere M, Zhang FP, Poutanen M
Scientific reports 2017 Nov 27;7(1):16406

Transcriptome Analysis of WHV/c-myc Transgenic Mice Implicates Cytochrome P450 Enzyme 17A1 as a Promising Biomarker for Hepatocellular Carcinoma.

Wang F, Huang J, Zhu Z, Ma X, Cao L, Zhang Y, Chen W, Dong Y
Cancer prevention research (Philadelphia, Pa.) 2016 Sep;9(9):739-49

Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.

Hakkarainen J, Jokela H, Pakarinen P, Heikelä H, Kätkänaho L, Vandenput L, Ohlsson C, Zhang FP, Poutanen M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Sep;29(9):3806-16

Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells.

Robitaille CN, Rivest P, Sanderson JT
Toxicological sciences : an official journal of the Society of Toxicology 2015 Jan;143(1):126-35

Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.

Tong A, Jia A, Yan S, Zhang Y, Xie Y, Liu G
International journal of clinical and experimental pathology 2014;7(7):4415-21

Increased expression of CYP17A1 indicates an effective targeting of the androgen receptor axis in castration resistant prostate cancer (CRPC).

Bremmer F, Jarry H, Strauß A, Behnes CL, Trojan L, Thelen P
SpringerPlus 2014;3:574

Intra-tissue steroid profiling indicates differential progesterone and testosterone metabolism in the endometrium and endometriosis lesions.

Huhtinen K, Saloniemi-Heinonen T, Keski-Rahkonen P, Desai R, Laajala D, Ståhle M, Häkkinen MR, Awosanya M, Suvitie P, Kujari H, Aittokallio T, Handelsman DJ, Auriola S, Perheentupa A, Poutanen M
The Journal of clinical endocrinology and metabolism 2014 Nov;99(11):E2188-97

Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.

Chrusciel M, Vuorenoja S, Mohanty B, Rivero-Müller A, Li X, Toppari J, Huhtaniemi I, Rahman NA
Journal of cell science 2013 Apr 15;126(Pt 8):1845-57

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 14447-1-AP(CYP17A1 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using CYP17A1 antibody 14447-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 14447-1-AP(CYP17A1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 14447-1-AP(CYP17A1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue