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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Immunohistochemistry [1]
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- Product number
- A00615-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Cytochrome P450 17A1/Cyp17a1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Cyp17a1 detection. Tested with WB, IHC-P, IF, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Long-term exposure to the mixture of phthalates induced male reproductive toxicity in rats and the alleviative effects of quercetin.
CYP17 gene plays a key role in goose genital growth by influencing the testosterone level at puberty.
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats.
Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells.
Liu LL, Yue JZ, Lu ZY, Deng RY, Li CC, Yu YN, Zhou WJ, Lin M, Gao HT, Liu J, Xia LZ
Toxicology and applied pharmacology 2024 Feb;483:116816
Toxicology and applied pharmacology 2024 Feb;483:116816
CYP17 gene plays a key role in goose genital growth by influencing the testosterone level at puberty.
Sun W, Gan X, Hu J, Li L, Wang J
Poultry science 2018 May 1;97(5):1748-1756
Poultry science 2018 May 1;97(5):1748-1756
Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats.
Gao HT, Xu R, Cao WX, Qian LL, Wang M, Lu L, Xu Q, Yu SQ
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Mar;101:94-104
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2017 Mar;101:94-104
Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells.
Liu C, Luan X, He Y, Xia X, Sun L, Miao W, Jin Y, Liu L
Biomedical microdevices 2014 Apr;16(2):209-16
Biomedical microdevices 2014 Apr;16(2):209-16
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody (A00615-3).Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cyp17a1 Antibody (A00615-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
