LS-C756411

antibody from LSBio

Targeting: CYP17A1 CPT7, CYP17, P450C17, S17AH

Western blot

ELISA

Antibody data

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Validation data

Product number: LS-C756411 - Provider product page
Provider: LSBio
Product name: CYP17 / CYP17A1 Antibody LS-C756411
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Mouse, Rat
Host: Rabbit
Isotype: IgG
Storage: After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

CYP17A1 protein structure - LS-C756411 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC analysis of Cyp17a1 using anti-Cyp17a1 antibody. Cyp17a1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-Cyp17a1 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.