Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [7]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BSM-54306R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome P450 17A1 Monoclonal Antibody (2F7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Specificity: This antibody reacts with both bone derived and platelet derived Osteonectin (ELISA). This antibody does not react with non-activated platelet and reacts with thrombin-stimulated platelet (flow cytometric analysis). This antibody reacts with human and bovine Osteonectin (Western blot analysis). This antibody cross reacts with rabbit and porcine Osteonectin. (ELISA)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2F7
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1:Mouse Adrenal gland lysates; Lane 2: Mouse Uterus lysates; Lane 3: Mouse Kidney lysates; Lane 4: Rat Testis lysates; Lane 5: Rat Adrenal gland lysates probed with Cytochrome P450 17A1 Monoclonal Antibody, Unconjugated (bsm-54306R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF (ICC) staining with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Breast tissue incubated with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SH=SY5Y cells with Cytochrome P450 17A1 (2F7) Monoclonal Antibody (bsm-54306R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
