Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-27777 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP17A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NIH-3T3, C2C12, MCF-7, HepG2. Predicted reactivity: Rhesus Monkey (91%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYP17A1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYP17A1 Polyclonal Antibody (Product # PA5-27777) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP17A1 Polyclonal Antibody (Product # PA5-27777). Sample (30 µg of whole cell lysate). Lane A: MCF-7 . 7.5% SDS PAGE. CYP17A1 Polyclonal Antibody (Product # PA5-27777) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYP17A1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYP17A1 Polyclonal Antibody (Product # PA5-27777) at a dilution of 1:1000.