Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- APR-024-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2X4 Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), C6 rat brain glioma cell line lysate (lanes 3 and 7) and SH-SY5Y human brain neuroblastoma cell line lysate (lanes 4 and 8): - 1-4. Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (1:200).5-8. Anti-P2X4 Receptor (extracellular) Antibody , preincubated with P2X4 Receptor (extracellular) Blocking Peptide (#BLP-PR024).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of P2RX4 in rat pheochromocytoma cell line (PC12) - Cell surface detection of P2RX4 in intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 (extracellular) Antibody (#APR-024), (red, 1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofP2X4by indirect flow cytometry in live intacthuman THP-1monocyticleukemiacells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (2.5μg) + goat- Anti-rabbit-FITC.