Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-60662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LBX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKRDVEEMRA DVASLRALSP EVLCSLALPE GAPDPGLCLG PAGPDSRPHL SDEEIQVDD
- Concentration
- 0.2 mg/mL
Submitted references LBX2-AS1/miR-219a-2-3p/FUS/LBX2 positive feedback loop contributes to the proliferation of gastric cancer.
Identification of LBX2 as a novel causal gene of lung adenocarcinoma.
Yang Z, Dong X, Pu M, Yang H, Chang W, Ji F, Liu T, Wei C, Zhang X, Qiu X
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2020 May;23(3):449-463
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2020 May;23(3):449-463
Identification of LBX2 as a novel causal gene of lung adenocarcinoma.
Hu J, Bai Y, Zhang Q, Li M, Yin R, Xu L
Thoracic cancer 2020 Aug;11(8):2137-2145
Thoracic cancer 2020 Aug;11(8):2137-2145
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LBX2 in human cell line CACO-2 shows positivity in cytokinetic bridge & nucleus but excluded from the nucleoli. Samples were probed using a LBX2 Polyclonal Antibody (Product # PA5-60662).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LBX2 in human cell line CACO-2 using a LBX2 Polyclonal Antibody (Product # PA5-60662) shows localization to nucleoplasm and midbody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LBX2 in human liver tissue shows moderate cytoplasmic positivity in hepatocytes. Samples were probed using a LBX2 Polyclonal Antibody (Product # PA5-60662).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1 Figure LBX2 is overexpressed in lung adenocarcinoma and correlates with clinicopathological characteristics. ( a ) LBX2 was hyperexpressed in LUAD tissues compared with adjacent normal tissues in the TCGA database ( P < 0.0001). ( b ) Overexpression of LBX2 in LUAD tissues were detected by immunohistochemistry (IHC). ( c ) Lung adenocarcinoma patients with low expression of LBX2 have a higher percentage of overall survival compared with high LBX2 expression patients ( P = 0.0075) ( ) LBX2-low, ( ) LBX2-high. ( d ) Compared with T1 tumors, LBX2 was significantly upregulated in T2, T3, and T4 tumors ( P = 0.0136). ( e ) N1-2 tumors had higher expression of LBX2 than those with N0 tumors ( P = 0.005) ( f ) LBX2 showed higher expression in tumors at a more advanced TNM stage ( P = 0.030).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure LBX2 promotes proliferation, migration and invasion of lung adenocarcinoma in vivo. ( a ) Xenograft tumors obtained from sh-LBX2 transfected A549 cell-treated mice had smaller tumors than the control group and tumors from OE-LBX2 had larger tumors than the control group. ( b ). IHC of tumor nodules shown LBX2 was downregulated in sh-LBX2 cells and upregulated in OE-LBX2 cells. ( c ) The volume and weight of tumor nodules of each group were measured and the results revealed that LBX2 promoted proliferation of LUAD in vivo. ( d,e ). Knockdown of LBX2 decreased metastatic activity to lung, liver and kidney tissues from subcutaneous xenografts, whereas overexpression of LBX2 greatly enhanced metastatic activity to lung, liver and kidney tissues ( ) lung, ( ) liver, ( ) kidney.
