TA800231

antibody from OriGene

Targeting: MEIS3 DKFZp547H236, MRG2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800231

Provider

OriGene

Proper citation

Product name

MEIS3 (Meis homeobox 3) mouse monoclonal antibody, clone OTI2E12 (formerly 2E12)

Antibody type

Monoclonal

Description

MEIS3 (Meis homeobox 3) mouse monoclonal antibody, clone OTI2E12 (formerly 2E12)

Host

Mouse

Conjugate

Unconjugated

Epitope

MEIS3

Isotype

IgG

Antibody clone number

OTI2E12

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEIS3 (RC206100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEIS3.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MEIS3 mouse monoclonal antibody (TA800231) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MEIS3(RC206100).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MEIS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800231)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MEIS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800231)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MEIS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800231)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206100 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MEIS3 antibody(TA800231), and then analyzed by flow cytometry.

Validation comment

FC