LS-B10591

antibody from LSBio

Targeting: TUBA1B K-ALPHA-1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B10591

Provider

LSBio

Product name

IHC-plus™ TUBA1B / Tubulin Alpha 1B Antibody (C-Terminus, clone 17H11.F10) LS-B10591

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

17H11.F10

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Western Blot. HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 ug of lysate each. The blot was blocked with Blocking Buffer (MB-070) for 30 min at RT, then washed and incubated with anti-Tubulin monoclonal antibody diluted in Blocking Buffer (p/n MB-070) at 1:1000 for 1 h at RT. After washing, blot was incubated with a 1:40000 dilution of HRP Rb a-Ms IgG (p/n LS-C60772) secondary antibody in Blocking Buffer (p/n MB-070) for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc 4000 MP.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.