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- Immunoprecipitation [1]
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- Product number
- BM753S - Provider product page
- Provider
- OriGene
- Product name
- Tubulin (TUBA1B) (Loading Control) mouse monoclonal antibody, clone TU-01, Purified
- Antibody type
- Monoclonal
- Description
- Tubulin (TUBA1B) (Loading Control) mouse monoclonal antibody, clone TU-01, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TUBA1B
- Isotype
- IgG
- Antibody clone number
- TU-01
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
Submitted references Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins.
Linhartová I, Dráber P, Dráberová E, Viklický V
The Biochemical journal 1992 Dec 15;288 ( Pt 3):919-24
The Biochemical journal 1992 Dec 15;288 ( Pt 3):919-24
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 4. Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 3. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green). Nucleus is stained with DAPI (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2. Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-Tubulin antibody (TU-01; red). Nucleus is stained with DAPI (blue).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green) and anti-Vimentin antibody (VI-01; SM3128P; red). Nucleus is stained with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 5. Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
- Validation comment
- IP
