PAB9934

antibody from Abnova Corporation

Targeting: TUBA1B K-ALPHA-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9934

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9934, RRID:AB_1716610

Product name

TUBA1B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of TUBA1B.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Structural features and restricted expression of a human alpha-tubulin gene.

Hall JL, Cowan NJ
Nucleic acids research 1985 Jan 11;13(1):207-23

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Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.

Cowan NJ, Dobner PR, Fuchs EV, Cleveland DW
Molecular and cellular biology 1983 Oct;3(10):1738-45

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using TUBA1B polyclonal antibody (Cat # PAB9934) shows detection of a predominant band at ~50 KDa corresponding to TUBA1B (arrowhead).Lanes correspond to whole cell lysates from mouse brain (Lane 1), rat brain (Lane 2), A-431 cells (Lane 3), Jurkat cells (Lane 4) and HeLa cells (Lane 5).Approximately 35 &mciro;g of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1 : 1,200.Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature.IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.