LS-C776139

antibody from LSBio

Targeting: TUBA1B K-ALPHA-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C776139

Provider

LSBio

Product name

TUBA1B / Tubulin Alpha 1B Antibody (Atto 633) LS-C776139

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human

Host

Rabbit

Conjugate

Red dye

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite.