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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- Ab179938 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- alpha Tubulin Mouse mAb
- Antibody type
- Monoclonal
- Description
- ApplicationWB: 1/1,000-1/20,000; Predicted molecular weight: 50 kDaFlow: 0.032 -1.0 _g/mLICC/IF: 2.0 _g/mL
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- AB01/5A12
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- alpha Tubulin Mouse mAb (Ab179938) - Western Blot All lanes: alpha Tubulin Antibody (Ab179938) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 50.0 kDa Observed band size: 50.0 kDa Exposure time: 8.8 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- alpha Tubulin Mouse mAb (Ab179938) - ICC/IF IF analysis of alpha Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- alpha Tubulin Mouse mAb (Ab179938) - ICC/IF IF analysis of alpha Tubulin (Green) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- alpha Tubulin Mouse mAb (Ab179938) - ICC/IF IF analysis of alpha Tubulin (Red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179006) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- alpha Tubulin Mouse mAb (Ab179938) - Flow Cytometry Overlay histogram showing HeLa cells stained with alpha Tubulin Mouse mAb (Ab179938) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).
