Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AMM06270 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- α-tubulin(Acetyl Lys40)(4A8)Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purification
- Reactivity
- Human, Mouse, Rat, Other
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Hela cells, untreated （-） or treated with TSA （1μM, 18 hr; +）, using Acetyl- a-tubulin（Lys40） Mouse mAb 1:2000.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Human-liver-cancer tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Mouse-lung tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Rat-spinal-cord tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,α-tubulin （Acetyl Lys40） Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin（Acetyl Lys40） Mouse mAb diluted at 1:200.
