Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP09289PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09289PU-N, RRID:AB_10529451
- Product name
- anti alpha Tubulin / TUBA1B (427-441) (Loading Control)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1.08 mg/ml (by UV absorbance at 280 nm)
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-alpha Tubulin antibody shows detection of a predominant band at ~50 kDa corresponding to alpha tubulin (arrowhead). Lanes correspond to whole cell lysates from mouse brain (lane 1), rat brain (lane 2), A431 cells (lane 3), Jurkat cells (lane 4) and HeLa cells (lane 5). Approximately 35 µg of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,200. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature. IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.