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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-96581 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MASVHESLYF NPMMTNGVVH ANVFGIKDWV TPYKIAVLVL LNEMSRTGEG AVSLMERRRL NQLLLPLLQG PDITLSKLYK LIEESCPQLA NSVQIRIKLM AEGELKDMEQ FFDDLSDSFS GTEPEVHKTS VVGLFLRHMI LAYSKLSFSQ VFKLYTALQQ YFQNGEKKTV EDADMELTSR DEGERKMEKE ELDVSVREEE VSCSGPLSQK QAEFFLSQQA SLLKNDETKA LTPASLQKEL NNLLKFNPDF; Positive Samples: SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of APC5 in 200 μg extracts of MCF-7 cells. Samples were precipitated with 3 μg APC5 Polyclonal antibody (Product # PA5-96581). Western blot was performed from the immunoprecipitate using APC5 Polyclonal antibody (Product # PA5-96581) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APC5 in paraffin-embedded rat kidney. Samples were incubated with APC5 Polyclonal antibody (Product # PA5-96581) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APC5 in paraffin-embedded human kidney. Samples were incubated with APC5 Polyclonal antibody (Product # PA5-96581) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of APC5 was performed in 200 µg extracts of MCF-7 cells using APC5 Polyclonal Antibody (Product # PA5-96581). Western blot was performed from the immunoprecipitate using APC5 Polyclonal Antibody.
