LS-C678638

antibody from LSBio

Targeting: MEIS1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C678638

Provider

LSBio

Product name

MEIS1 Antibody LS-C678638

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, Mouse skeletal muscle tissue All lanes: MEIS1 antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 51 kDa Observed band size: 44 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in:Jurkat whole cell lysate,K562 whole cell lysate,Hela whole cell lysate,Mouse skeletal muscle tissue All Lanes: MEIS1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44,51 kDa Observed band size: 44 kDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in:Jurkat whole cell lysate,K562 whole cell lysate,Hela whole cell lysate,Mouse skeletal muscle tissue All Lanes: MEIS1 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44,51 kDa Observed band size: 44 kDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, Mouse skeletal muscle tissue All lanes: MEIS1 antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 51 kDa Observed band size: 44 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating MEIS1 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MEIS1 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MEIS1 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of MEIS1 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: MEIS1 Antibody (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MEIS1 in K562 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of product in K562 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the Secondary antibody (1/50000) Lane 2: product (4µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)