Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-23376 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEIS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MEIS1 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in MEIS1 polyclonal antibody (Product # PA5-23376) using a dilution of 3 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MEIS1 Polyclonal Antibody, (Product # PA5-23376) and ~53, 50 kDa bands corresponding to isoforms of MEIS1 were observed across tissues tested. Whole cell extracts (30 µg lysate) of Mouse Lung (Lane 1), Mouse Brain (Lane 2), Mouse Kidney (Lane 3), Mouse Placenta (Lane 4), Mouse Skeletal Muscle (Lane 5) (reported to be negative) and Mouse Spleen (Lane 6) (reported to be negative) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MEIS1 in formalin-fixed, paraffin-embedded human brain tissue. Samples were incubated in MEIS1 polyclonal antibody (Product # PA5-23376) using a dilution of 5 µg/mL. Isotype control (top left) and this antibody (bottom left, right).