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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77903 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77903, RRID:AB_11018209
- Product name
- Rabbit Polyclonal RNF34 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. This protein A purified antibody is directed against human RNF34 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RNF34 Antibody [NBP1-77903] - Shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 ul of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
