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Learn16365-1-AP
antibody from Invitrogen Antibodies
Targeting: HNRNPU
C1orf199, FLJ30202, FLJ37978, HNRNPU-AS1, HNRPU, NCRNA00201, SAF-A
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 16365-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HNRNPU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LEDRVRGPK GPEEKKDCEV VMMIGLPGAG KTTWVTKHAA ENPGKYNILG TNTIMDKMMV AGFKKQMADT GKLNTLLQRA PQCLGKFIEI AARKKRNFIL DQTNVSAAAQ RRKMCLFAGF QRKAVVVCPK DEDYKQRTQK KAEVEGKDLP EHAVLKMKGN FTLPEVAECF DEITYVELQK EEAQKLLEQY KEESKKALPP EKKQNTGSKK SNKNKSGKNQ FNRGGGHRGR GGFNMRGGNF RGGAPGNRGG YNRRGNMPQR GGGGGGSGGI GYPYPRAPVF PGRGSYSNRG NYNRGGMPNR GNYNQNFRGR GNNRGYKNQS QGYNQWQQGQ FWGQKPWSQH YHQGYY (462-806 aa encoded by BC003367)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16365-1-AP (HNRNPU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16365-1-AP (HNRNPU antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 16365-1-AP (HNRNPU antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug HNRNPU antibody (16365-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HNRNPU (IP:16365-1-AP, 3ug; Detection:16365-1-AP 1:800) with HeLa cells lysate 2000ug.
