PA5-78481

antibody from Invitrogen Antibodies

Targeting: C11orf54 PTD012

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78481

Provider

Invitrogen Antibodies

Product name

C11orf54 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, H1299, HCT116

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C11orf54 in A) A549 whole cell lysate, B) H1299 whole cell lysate, C) HCT116 whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with C11orf54 polyclonal antibody (Product # PA5-78481) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C11orf54 Polyclonal Antibody detects C11orf54 protein by Western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HCT116 whole cell lysate/extract.12 % SDS-PAGE. C11orf54 Polyclonal Antibody (Product # PA5-78481) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of C11orf54 in HCT116 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with C11orf54 polyclonal antibody (Product # PA5-78481) using a dilution of 1:2000, followed by Hoechst.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C11orf54 Polyclonal Antibody detects C11orf54 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: C11orf54 protein stained by C11orf54 Polyclonal Antibody (Product # PA5-78481) diluted at 1:2,000. Blue: Hoechst 33342 staining.