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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-78481 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C11orf54 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, H1299, HCT116 Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C11orf54 in A) A549 whole cell lysate, B) H1299 whole cell lysate, C) HCT116 whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with C11orf54 polyclonal antibody (Product # PA5-78481) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C11orf54 Polyclonal Antibody detects C11orf54 protein by Western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HCT116 whole cell lysate/extract.12 % SDS-PAGE. C11orf54 Polyclonal Antibody (Product # PA5-78481) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C11orf54 in HCT116 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with C11orf54 polyclonal antibody (Product # PA5-78481) using a dilution of 1:2000, followed by Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C11orf54 Polyclonal Antibody detects C11orf54 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: C11orf54 protein stained by C11orf54 Polyclonal Antibody (Product # PA5-78481) diluted at 1:2,000. Blue: Hoechst 33342 staining.
