10970-1-AP
antibody from Invitrogen Antibodies
		Targeting: PABPC1
		
		PAB1, PABP1, PABPC2, PABPL1	
	
	
	
	Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [6]
 - Immunocytochemistry [2]
 - Immunohistochemistry [4]
 - Flow cytometry [1]
 - Other assay [1]
 
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- Product number
 - 10970-1-AP - Provider product page

 - Provider
 - Invitrogen Antibodies
 - Product name
 - PABPC1/PABP Polyclonal Antibody
 - Antibody type
 - Polyclonal
 - Antigen
 - Other
 - Reactivity
 - Human, Mouse, Rat
 - Host
 - Rabbit
 - Isotype
 - IgG
 - Vial size
 - 150 µL
 - Concentration
 - 0.33 mg/mL
 - Storage
 - -20°C
 
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					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HeLa cells were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Rat testis tissue were subjected to SDS PAGE followed by western blot with 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunofluorescent analysis of MCF-7 cells, using PABPC1 antibody 10970-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 40x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human pancreas using 10970-1-AP (PABPC1,PABP antibody) at dilution of 1:50 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human testis tissue slide using 10970-1-AP (PABPC1,PABP Antibody) at dilution of 1:50.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - 1X10^6 MCF-7 cells were stained with 0.2ug PABPC1,PABP antibody (10970-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - IP result of anti-PABPC1,PABP (IP:10970-1-AP, 4ug; Detection:10970-1-AP 1:700) with mouse testis tissue lysate 4000ug.