- PA5-29883 - Invitrogen Antibodies PABPC1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of PABP using 30 µg of A) A549 (B) H1299 (C) HCT116 and D) MCF-7 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PABP polyclonal antibody (Product # PA5-29883) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PABP was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PABP Polyclonal Antibody (Product # PA5-29883) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), MCF7 (Lane 3), NTERA-2 (Lane 4), NIH/3T3 (Lane 5), RAW 264.7 (Lane 6), C2C12 (Lane 7) and HeLa (Lane 8). The blot was probed with Anti-PABP Polyclonal Antibody (Product # PA5-29883, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to PABP was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PABP was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PABP Polyclonal Antibody (Product # PA5-29883) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PABP Polyclonal Antibody (Product # PA5-29883). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP Polyclonal Antibody (Product # PA5-29883) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PABP was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PABP Polyclonal Antibody (Product # PA5-29883) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PABP was performed by separating 30 µg of various whole cell lysates by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a PABP Polyclonal Antibody (Product # PA5-29883) at a dilution of 1:1000. A. 293T, B. A431, C. HeLa, D. HepG2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PABP was performed by separating 30 µg of whole cell lysates by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a PABP Polyclonal Antibody (Product # PA5-29883) at a dilution of 1:1000. A. A549, B. H1299.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of PABP was achieved by transfecting HCT 116 cells with PABP specific siRNAs (Silencer® select Product # s25666, s25664). Western blot analysis (Fig. a) was performed using whole cell extracts from the PABP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PABP Polyclonal Antibody (Product # PA5-29883, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PABP.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: PABP Polyclonal Antibody detects PABP protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABP protein stained by PABP Polyclonal Antibody (Product # PA5-29883) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Rabbit Polyclonal Antibody(Product # PA5-29883) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABP (Product # PA5-29883) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: PABP Polyclonal Antibody detects PABP protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PABP Polyclonal Antibody (Product # PA5-29883) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: PABP Polyclonal Antibody detects PABP protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PABP Polyclonal Antibody (Product # PA5-29883) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: PABP Polyclonal Antibody detects PABP protein at cytosol on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PABP Polyclonal Antibody (Product # PA5-29883) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection of binding of endogenous PABP protein to specific RNA using Anti-PABP Antibody: RNA Immunoprecipitation (RIP) was performed using Anti-PABP Polyclonal Antibody (Product # PA5-29883, 4 µg) on clarified whole cell lysate from 2 million Hep G2 cells. Normal Rabbit IgG was used as a negative IP control. Immunoprecipitated RNA was purified by RiboPure™ RNA Purification Kit (Product # AM1924) and analyzed by RT-PCR using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Product # 4389986) with primer pairs over ACTB mRNA, GAPDH mRNA (positive) and U1 snRNA, 18S rRNA (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

PA5-29883

Antibody data