MA5-42771

antibody from Invitrogen Antibodies

Targeting: SMURF2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42771

Provider

Invitrogen Antibodies

Product name

SMURF2 Recombinant Rabbit Monoclonal Antibody (8O7F0)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: U2OS, MCF7, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain. The target is usually found in the following locations: Cell membrane, Cytoplasm, Membrane raft, Nucleus. Immunogen sequence: PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

8O7F0

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SMURF2 in extracts of various cell lines. Samples were incubated with SMURF2 Monoclonal antibody (Product # MA5-42771) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SMURF2 in NIH-3T3 cells using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SMURF2 in NIH-3T3 cells using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SMURF2 in NIH-3T3 cells. Samples were incubated with SMURF2 Monoclonal antibody (Product # MA5-42771) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of SMURF2 in 300 μg extracts of Mouse lung. Samples were precipitated with 3 μg SMURF2 Monoclonal antibody (Product # MA5-42771). Western blot was performed from the immunoprecipitate using SMURF2 Monoclonal antibody (Product # MA5-42771) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SMURF2 in paraffin-embedded human colon. Samples were incubated with SMURF2 Monoclonal antibody (Product # MA5-42771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SMURF2 in paraffin-embedded human colon. Samples were incubated with SMURF2 Monoclonal antibody (Product # MA5-42771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.