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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-42771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMURF2 Recombinant Rabbit Monoclonal Antibody (8O7F0)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: U2OS, MCF7, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain. The target is usually found in the following locations: Cell membrane, Cytoplasm, Membrane raft, Nucleus. Immunogen sequence: PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 8O7F0
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMURF2 in Mouse lung cells using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMURF2 in various cell lines using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SMURF2 in NIH-3T3 cells using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SMURF2 in NIH-3T3 cells using a SMURF2 Recombinant Monoclonal antibody (Product # MA5-42771). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SMURF2 in paraffin-embedded human colon. Samples were incubated with SMURF2 Monoclonal antibody (Product # MA5-42771) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
